MHC tetramers for detection of OT-I cells that are SIINFEKL / Ova specific T cells
Ovalbumin (OVA) is a T Cell-dependent antigen that is frequently used as a model protein for studying antigen-specific immune responses in ovalbumin sensitized C57BL/6 mice or in TCR transgenic OT-I mice.
The murine T cell response to OVA is well characterized. Proteolytic processing of ovalbumin generates ovalbumin-derived peptide fragments of 8-10 amino acids including the immune-dominant SIINFEKL (Ser – Ile – Ile – Asn – Phe – Glu – Lys – Leu) (OVA 257-264) peptide responsible for eliciting a CD8+ antigen-specific T Cell response (also termed OT-I cells).
This ovalbumin-derived peptide fragment is displayed in the context of the MHC class I molecule H-2Kb specific for C57BL/6 mice. The resulting presentation of H-2Kb/SIINFEKL to T cells prompt a well-defined cellular immune response in these mice. Since the identification of SIINFEKL as the immune-dominant OVA peptide, numerous studies have described the use of H-2Kb/SIINFEKL MHC Tetramers.
MHC Tetramer for detection of OVA/SIINFEKL-specific T cells >>To order press here
|Cat. No.||MHC type||Pep. Seq.||Antigen|
Specification of Tetramers
Tetramer Shop supply MHC Tetramers in various test sizes and the colors PE, BV421, BUV395, APC-Cy™7, APC, BV510, BV480, PE-Cy™5, PE-CF594, PE-Cy™7, PerCP-Cy™5.5, PerCP, FITC, BV605, BV650, BV786, APC-R700, BB515 and BUV737. For other colors, please enquire.