T cells are enumerated and characterized by MHC tetramer assays or simply tetramer assays or tetramer stains.
However, the inherent instability of the MHC complex has made tetramer assays challenging, expensive and inflexible assays.
Tetramer Shop has developed a new stabilized form of the MHC complex that allows for large-scale production of peptide-empty MHC monomers that can be quality controlled before loading peptide antigen into the empty MHC monomer.
This allows for unprecedented speed of production and delivery as well as broadest range of available colors.
Furthermore, we offer Empty Loadable Tetramers that can be instantly loaded with peptide antigen (see protocol) by the customer, essentially representing DIY tetramers or instant tetramers on demand. No UV-induced peptide exchange, no bleaching – just add peptide and stain.
Empty Loadable Tetramers represent a major improvement in flexibility and quality for tetramer assays.
To order Empty Loadable Tetramers from Tetramer Shop >> Press Here
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What can Empty Loadable Tetramers be used for?
Empty Loadable Tetramers can be used just like conventional Tetramers. However, they are supplied empty of peptide antigen and you have to add peptide before you can stain your T cells.
What is the advantage of Empty loadable Tetramers?
Besides the improved stability and brighter staining the major advantage is that you can instantly DIY your own Tetramer specificities just by adding a specific peptide antigen (See protocol). This is a dramatic increase in flexibility and can decrease your Tetramer expenses significantly.
The flexibility of Empty Loadable Tetramers make these reagents ideal for multi-color flow cytometry or combinatorial encoding and detection of multiple T cell specificities in a single sample. This is in particular advantageous when you are staining for multiple T cell specificities for neoantigens or proprietary peptide antigens.
Tetramers for immunotherapeutic development.
Immunotherapy and vaccine development is largely dependent on tetramer technologies for epitope detection, monitoring of disease and progression of disease, monitoring of immunotherapeutic effect on disease-specific T cells and the aforementioned isolation, expansion and re-introduction of disease specific T cells into the patient.
Advantages of Tetramer assays
Tetramer assays represent an important improvement over other procedures, such as ELISPOT and single-cell PCR because they allow physical isolation of the cells by fluorescence-activated cell sorter (FACS) and subsequent further study of sorted cells. As a flow-cytometry-based application, tetramers are also easy to use and have a short assay time. Tetramers also allow the antigen specific immune response to be analyzed in both animal models and in man.
Tetramer Shop supply MHC Tetramers in various test sizes and the colors PE, BV421, BUV395, APC-Cy™7, APC, BV510, BV480, PE-Cy™5, PE-CF594, PE-Cy™7, PerCP-Cy™5.5, PerCP, FITC, BV605, BV650, BV786, APC-R700, BB515 and BUV737. For other colors, please enquire.