|Cat. No||**Catalog No. inserted here**|
|Product supplied||CD1d Tetramer conjugated with fluorochrome.|
|MHC allele||**Allele inserted here**|
|Peptide||**Peptide inserted here**|
|Category||**Category inserted here**|
|Dye/fluorochrome||**Dye inserted here**|
|Size||**Size inserted here**|
|Formulation||PBS, pH 7.4 containing, 0,5% BSA, 5% glycerol and < 0.01% NaN3. A 50 test size contains 250 µL MHC Tetramer reagent. One test is 5 µl. One test contains 0.2 µg CD1d monomer conjugated to 0.05 µg streptavidin with relevant fluorochrome.|
|Recommended use||Recommended for detection of NKT cells by flow cytometry.|
|Precautions||The product is not for clinical use. For research use only (RUO).
The product contains sodium azide (NaN3). At product concentrations, though not classified as hazardous, sodium azide may react with lead and copper plumbing to form highly explosive build-ups of metal azides. Upon disposal, flush with large volumes of water to prevent metal azide build-up in plumbing. Please see MSDS.
|Storage||Store at -20°C if not used the same day. Do not repeatedly freeze/thaw. Can be stored at 4°C during handling.|
|Approved date||**Todays date inserted here**|
|Background||CD1d Tetramers recognize NKT cells that are specific for a particular lipid antigen in context of CD1d. All specific NKT cells regardless of their functional status are detected using CD1d Tetramers.|
|Lipid loading||This procedure successfully loads Alpha-Galactosyl Ceramide into ligand-empty human and mouse CD1d Tetramers. Optimal conditions and molar stoichiometry will need optimization for loading other lipid antigens.
This procedure does not apply for CD1d Tetramers already loaded with Alpha-GalCer.Materials:
Procedure for lipid loading of 10 tests of empty CD1d tetramers:
|Staining procedure||Procedure for staining NKT cells with CD1d Tetramers.
Avoid bright or direct light due to light-sensitive fluorochromes.
It is important that cells are incubated with CD1d Tetramer reagent 10 min. prior to addition of anti-CD8 antibody for optimal Tetramer staining.